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Renuga Devi, T. S.
- Analysis on Renal Failure Patients Blood Samples: Characterization and Efficacy Study
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PDF Views:122
Authors
Affiliations
1 PG Department of Physics, Women’s Christian College, Chennai, IN
2 Periyar University, Salem, IN
3 Department of Mathematics, Women’s Christian College, IN
1 PG Department of Physics, Women’s Christian College, Chennai, IN
2 Periyar University, Salem, IN
3 Department of Mathematics, Women’s Christian College, IN
Source
Indian Journal of Science and Technology, Vol 2, No 2 (2009), Pagination: 46-50Abstract
This study attempts to evaluate the spectral difference between healthy and renal failure blood sera and also to find the efficacy of peritoneal dialysis in renal failure patients using FTIR spectroscopic technique. Six chronic renal failure patients were enrolled in the study. FTIR blood spectra were recorded over the region 4000 - 400 cm -1 on a Perkin Elmer spectrum one FTIR spectrometer. The internal standard ratios among the absorption peaks were calculated and it is found to be an indicator to differentiate the diseased subjects from the healthy one. In order to find the efficacy of peritoneal dialysis the absorption values of the specific bands of both pre and post treatment spectra were noted and the internal ratio parameter were calculated. The comparison of these values showed that the spectra are not similar when compared down to finer details.Keywords
Blood, Renal Failure, Peritoneal Dialysis, FTIR SpectroscopyFull Text
References
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- A Comparative Study of Pre and Post Vaccinated Sera Samples of Bovine Using FTIR Spectral Analysis
Abstract Views :952 |
PDF Views:140
Authors
Affiliations
1 Sri Chandrasekharendra Saraswathi Viswa MahaVidyalaya, Enathur, Kanchipuram-631 561, Tamilnadu, IN
2 Registrar, Periyar University, Salem-636 011, Tamilnadu, IN
3 P.G.Department of Physics, Women’s Christian College, Chennai-600 005, Tamilnadu, IN
1 Sri Chandrasekharendra Saraswathi Viswa MahaVidyalaya, Enathur, Kanchipuram-631 561, Tamilnadu, IN
2 Registrar, Periyar University, Salem-636 011, Tamilnadu, IN
3 P.G.Department of Physics, Women’s Christian College, Chennai-600 005, Tamilnadu, IN
Source
Indian Journal of Science and Technology, Vol 3, No 4 (2010), Pagination: 382-386Abstract
This study provides scope for spectroscopic method that can be used as in-vitro test to assess the potent of vaccination instead of conventional in-vivo test. India tops in cattle wealth as well as in milk production in the world. Livestock are to be regularly vaccinated to protect them from many infectious and contagious diseases. We investigated the changes in protein and lipids levels in the pre and post vaccinated sera samples of bovine using FTIR spectral analysis. Two groups of seven animals were vaccinated with Haemorrhagic Septicaemia Vaccine (HSV) and Black Quarter Vaccine (BQV). Pre and post vaccinated sera samples of cattle were collected and tested using Fourier Transform Infra Red (FTIR) spectrometer. Spectroscopic method of blood analysis is an alternate technique to the clinical method since they require fewer samples and provide more information. The variation observed in peaks indicating the change in protein and lipids levels in the animals was due to introduce of antigens. Compared to ELISA, spectral analysis is cost effective test besides it requires small amount of sample for analysis and conforms to bioethics. In vivo challenge test can be replaced once this procedure is standardized which can satisfy the CPCSEA-"Committee for the Purpose of Control and Supervision on Experiments on Animal"- which imposes stringent regulations to use animal for experiments.Keywords
Cattle, Livestock, Vaccination, Spectroscopy, FTIR SpectrometerFull Text
References
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- Gunasekaran .S and T.S.Renuga Devi (2008) Analysis of Hyperlipidemic Patients Blood Samples – Spectral and Statistical Implications. Asian J. Clinical Cardiology, 10 (10),14-19.
- Gunasekaran S and Sankari G (2004) FTIR and UV-Visible spectral study on normal and diseased blood samples. Asian J. Chem. 16, 1779-1786.
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- Lingathurai S, Vellathurai P, Ezil Vendan S and Alwin Prem Anand A (2009) A comparative study on the microbiological and chemical composition of cow milk from different locations in Madurai, Tamil Nadu. Indian J.Sci.Technol. 2 (2), 51-54. Domain site: http://www.indjst.org.
- Renuga Devi TS, Gunasekaran S, Wesley Hudson J and Sarone Angelah Joybell I (2009) Analysis on renal failure patients blood samples: characterization and efficacy study. Indian J.Sci.Technol. 2 (2), 46-50. Domain site: http://www.indjst.org.
- Shaw RA, Kotowich S, Leroux M and Mantsch HH (1998) Multianalyte serum analysis using mid-infrared spectroscopy. Ann. Clin. Biochem. 35, 624-632.
- Srinivasan VA, Reddy GS, Rao KA and Ueli Kihm (2001) Serological response of bovines to combined vaccine containing foot and mouth disease virus, rabies virus, Pasteurella multocida and Clostridium chauvoei antigens. Veterinarski Arhiv. 71 (1), 37-45.